Form Cs 4211 Material Plant Book Table Of Contents Pennsylvania
Form Cs 4211 Material Plant Book Table Of Contents Pennsylvania – Genetic variation of native linen germplasm collected from South Korea using simple sequence repeat (SSR) markers and morphological traits.
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Form Cs 4211 Material Plant Book Table Of Contents Pennsylvania
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By Bowei Cai, Tai Wang, Wenqin Fu, Arrashid Haroon, Xianhong Ge * and Xiayun Li
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National Key Laboratory of Crop Genetic Improvement, National Oilseed Improvement Center (Wuhan), College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
Received: 21 July 2021 / Revised: 20 August 2021 / Accepted: 20 August 2021 / Published: 25 August 2021
Distant hybridization usually results in sterility of the female hybrid, but the mechanism behind this is poorly understood. Complete pistil abortion but normal male fertility was shown by a monosomic outbred addition line Brassica napus-Orychophragmus violaceus (MA, AACC + 1 I).
, 2n = 39), created earlier. A single O. on pistil development in different genetic environments. To study the effect of adding purple chromosomes, M.A. and b. carinata (B.B.C.), r. The first hybrids between Junsia (AABB) and two synthetic hexaploids (AABBCC) were produced. This study shows complete sterilization of women. Further, microspore culture was performed to generate a haploid monosomic foreign addition line (HMA, AC + 1 I).
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, 2n = 40) with haploid (H, AC, 2n = 19) and double haploid (DH, AACC, 2n = 38) plants of B. MA from Naps Alien on gun from O. To study the dose effect of the violet chromosome. Development and gene expression. Compared with MA, pistil development was fully or partially restored in DA and HMA, with pistils able to swell and grow to normal size after open pollination, although seeds did not form. Comparative RNA-seq analyzes showed that the number of differentially expressed genes (DEGs) was significantly different depending on dose and HMA vs. H, DA vs. DH, MA vs. DH, DA vs. D. H. MA vs. DA and MA vs. HMA. Gene Ontology (GO) enrichment analysis of DEGs revealed that many genes are involved in the development of the gynoecium, embryo sac, ovary, and integuments. In particular, several DEGs common to pistil development in HMA vs. H and DA vs. DH showed functions in genotoxic stress response, auxin transport, and adaxial/axial axis signaling and specification. The results showed that Alien O. Violaceous chromosomes dose-responsive B. Provided updated information for the molecular mechanisms for napus gynoecium development.
Pistil development begins with one or more fused carpels that have developed from leaves . In many species of Brassicaceae, including the model plant Arabidopsis thaliana, the gynoecium consists of two fused carpels [2, 3]. At the edge where the two carpels fuse, the carpel margin meristem (CMM) is formed, and then the placenta, ovary, septum and reproductive tract are formed there . The egg develops from the cells of the egg primordium and forms a finger-like structure in the early developmental stage, which then differentiates into the funiculus, chalazae and nucellus . At the tip of the nucellus, the megaspore mother cell differentiates from the hypodermal cell and undergoes meiosis followed by three rounds of mitosis to form a mature embryo sac containing three antipodal cells, two medial polar nuclei, ovules, and two are synergized. cells . Pistil development is a complex process that requires multiple tissues to achieve a specific identity at specific locations. This process can be realized through a regulatory network formed by genes and hormones, including auxins, cytokinins (CK), gibberellins (GA) and brassinosteroids (BR) [7, 8]. Genes can influence hormone pathways at various levels, including biosynthesis, transport, and signaling, and in turn, hormones can influence the regulation of gene transcription.
Postzygotic reproductive isolation, such as hybrid sterility, hybrid necrosis/weakness, and hybrid lethality, is frequently observed in crop plants, leading to reduced fitness of hybrids among different species groups. These limitations are disadvantageous traits for the use of heterosis and are major obstacles to genetic improvement of yield. Hybrid sterility represents the most common form of postzygotic reproductive barrier in plants, and a well-characterized example is hybrid sterility between two subspecies of Asian cultivated rice (Oryza sativa L.) . According to the stage of development, female infertility can be divided into three types: one is that the pistil is not differentiated at all or is incomplete; The second is that the pistil lacks a normal embryo sac and a failed ovary; The third is that the pistil has a normal embryo sac, but the ovules do not develop into an embryo after pollination.
With the rapid development of sequencing technology, genome and transcriptome research is becoming easier and more feasible, as next-generation sequencing methods have dramatically increased sequencing efficiency and reduced sequencing costs [ 10 ]. Transcriptomic analysis is often used to study the overall level of gene expression of specific tissues, which is suitable for the overall analysis of the gene regulatory network of a certain character [11, 12, 13]. Transcriptome analysis of pistil abortion has been reported in several species including A. thaliana , Oryza sativa , Prunus mum , Brassica rapa , B. Naps [18, 19], including Oleo. europaea , Citrus reticulate  and Hieracium preeltum . All these studies show that many genes are involved in the regulation of pistil development and that embryo sac formation and sheath development are interdependent or partially controlled by a common pathway, indicating the complexity of pistil growth regulation. 23, 24, 25].
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Oilseed rape (B. napus, 2n = 38, AACC), an economically important oilseed, is extensively cultivated worldwide [26, 27]. Because of the value of hybrid seeds in agriculture, much research has focused on male sterility in rapeseed, but very few studies have been reported on pistil development in B. napus [ 28 , 29 ]. First we b. reported the female sterile plant Naps-o. Violaceous monosomic alien addition line (MA) . The results showed that female gametophyte development was arrested after the tetrad stage and before the onset of megagametogenesis . Here a single O. on pistil development in different genetic environments. To study the effect of addition of violaceus chromosomes, hybrids between MA and other Brassica species were produced and phenotypically observed. Meanwhile, Alien O. on pistil development and gene expression changes in Brassica napus by phenotypic and comparative transcriptome analysis. A haploid monosomic foreign addition line and a disomic addition line were generated from MA by microspore culture to investigate the dosage effect of purple chromosomes.
Female sterile b. naps-o B. in purple monosomic foreign addition lines (MA). Napus (2n = 38, AACC) complete chromosome complement and one chromosome from O. violaceus (2n = 24, OO) (AACC + 1 I)
, 2n = 39), previously developed from a somatic hybrid of these two species (2n = 62, AACCOO), was bred from the parental cultivar B. napus “Huashuang 3” (H3) [30, 31]. was reversed. We previously reported a female sterile disomic addition line with one pair of O. violaceus chromosomes (S1) . However, the line was later confirmed as a double monosomic addition with two different O. violaceus chromosomes (data not shown). Furthermore, since pairing of individual chromosomes was complete in sterile females of a different genomic background, disomic pairing could not be achieved by swelling.
Between MA and two synthetic Brassica hexaploids (AABBCC, 2n = 54) [32, 33] and Brassica juncea (AABB, 2n = 36) (accession number, GJ19) and B. carinata (accession number G0-7). were created by hand pollination.
Multiple Cyclic Nucleotide‐gated Channels Coordinate Calcium Oscillations And Polar Growth Of Root Hairs
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